Welcome Guest
Sign In
Register
Products
Popular Categories
Antibody Search
ELISA Kit Search
Biomolecules
Flow Cytometers
Thermal Cyclers
Microscopes
Transfection Reagents
Featured Products
New Products
Articles
Resources
eBooks
Bench Tips
Tech Insights
Editorial Articles
News
Product Reviews
Future Lab
Multimedia
Biocompare Webinars
Immunotherapy
Reproducibility
Transformative Technologies
Podcasts
Tech Talks
Bench Tip Videos
All Videos and Webinars
Life Science Webinars
Write a Review
Antibodies
Kits/Reagents
Equipment
Services
Resources
Promotions
Events
AACR SPOTLIGHT
Register
Sign In
Biocompare
Products
Cloning and Expression
Cell Culture / Tissue Culture
Cell Culture Media
Cell Culture Media and Supplements
Gelatin solution
Gelatin solution from MilliporeSigma
Be the first to write a review!
Citations:
(276)
Supplier Page
Supplier Page from
MilliporeSigma for
Gelatin solution
Description
This product is recommended for use as a cell culture substratum at 1-5 ?g/cm
2
or 0.5-50 ?g/mL. The optimal concentration does depend on cell type as well as the application and research objectives. Gelatin has been used in many appli
Product Specs
Company Info
Citations
Reviews
Product Specs
Item
Gelatin solution
Company
MilliporeSigma
Price
Pricing Info
Supplier Page
View Company Product Page
Catalog Number
G1393
Concentration
2% in H2O, tissue culture grade
Species
bovine hide
Applications
mammalian cell culture
Type
solution
Quantity
100ML, 20ML
Add to Compare List
MilliporeSigma
400 Summit Drive
Burlington
,
Massachusetts
01803
United States
Phone
: (800)-645-5476
(800)-645-5439
Company Profile
Website:
www.SigmaAldrich.com/
Citations (276)
(41)
Hybrid assembly of polymeric nanofiber network for robust and electronically conductive hydrogels
Nature Communications
February 10, 2023
Huimin He, Hao Li, Aoyang Pu, Wenxiu Li, Kiwon Ban, Lizhi Xu
(Gibco). For promoting cell attachment, samples were further treated with 0.1% gelatin solution (Type B,
G1393
Sigma
) under 37 °C for 1 h. CNH Neonatal
rat
cardiomyocytes
(NRCMs) were harvested from the left ventricles
(42)
Novel Green Fluorescent Polyamines to Analyze ATP13A2 and ATP13A3 Activity in the Mammalian Polyamine Transport System
Biomolecules
February 9, 2023
Marine Houdou, Nathalie Jacobs, Jonathan Coene, Mujahid Azfar, Roeland Vanhoutte, Chris Van den Haute, Jan Eggermont, Veronique Daniëls, Steven H. L. Verhelst, Peter Vangheluwe
195849;
ATP13A3
D498N, 195850; RRID: Addgene_82067). HMEC-1 cells were cultured in 0.2% gelatin (
Sigma
,
G1393
) coated 75 cm2 flasks and in MCDB131 culture medium without
L-glutamine
(
Gibco
: 10372-019), supplemented
(43)
Corneal wound healing and nerve regeneration by novel ophthalmic formulations based on cross-linked sodium hyaluronate, taurine, vitamin B6, and vitamin B12
Frontiers in Pharmacology
February 2, 2023
Claudio Bucolo, Grazia Maugeri, Salvatore Giunta, Velia D’Agata, Filippo Drago, Giovanni Luca Romano
air. Cell culture plates were coated with 5–10 µL gelatin solution/cm2 (i.e., 0.1–0.2 mg/cm2 gelatin,
G1393
,
Sigma-Aldrich
) to promote cell adhesion. SIRCs (P6) were cultured with or without test formulations.
(44)
MARCKSL1 interacted with F‐actin to promote esophageal squamous cell carcinoma mobility by modulating the formation of invadopodia
Cancer Medicine
February 1, 2023
Yue Zhao, Xiufeng Xie, Lusong Tian, Fang Liu, Yulin Sun, Haizhen Lu, Xiaohang Zhao, Yousheng Mao
reported. 17 In brief, the bottoms of glass dishes were first coated with 0.1% unconjugated gelatin (
G1393
,
Sigma
) and fluorescent gelatin (
1:5
dilution of Oregon green 488 conjugated gelatin [
G13186
,
Invitrogen
]).
(45)
Transcriptomic-based evaluation of trichloroethylene glutathione and cysteine conjugates demonstrate phenotype-dependent stress responses in a panel of human in vitro models
Archives of Toxicology
February 1, 2023
Liliana Capinha, Yaran Zhang, Anna-Katharina Holzer, Anna-Katharina Ückert, Melinda Zana, Giada Carta, Cormac Murphy, Jenna Baldovini, Zahra Mazidi, Johannes Grillari,
et al.
controlled humidified 37 °C, 5% CO2 environment in plates coated with 0.1% gelatin solution (
Sigma-Aldrich
G1393
) in phosphate-buffered saline solution (PBS). Cells were tested 1–2 days after reaching confluency in
(46)
An Engineered IFNγ-Antibody Fusion Protein with Improved Tumor-Homing Properties
Pharmaceutics
January 22, 2023
Di Nitto C, Gilardoni E, Mock J, Nadal L, Weiss T, Weller M, Seehusen F, Libbra C, Puca E, Neri D,
et al
.
was used for F9, THP-1 and TIB-49. F9 cells were grown on 0.1% gelatin-coated flasks (
Sigma-Aldrich
G1393
). RPMI (
Gibco
11875093
) + 10% FBS (Gibco 10270106) was used for CT26, WEHI-164. Authentication of the
(47)
Nesprin-1 LINC complexes recruit microtubule cytoskeleton proteins and drive pathology in Lmna-mutant striated muscle
Human Molecular Genetics
January 6, 2023
Ei Leen Leong, Nyein Thet Khaing, Bruno Cadot, Wei Liang Hong, Serguei Kozlov, Hendrikje Werner, Esther Sook Miin Wong, Colin L Stewart, Brian Burke, Yin Loon Lee
supernatant (with floating
myoblasts
) and replated into 60 mm dishes coated with 0.15% gelatin (
Sigma
, cat
G1393
). D10 medium was added to the 100 mm plates with MAFs. To terminally differentiate
myoblasts
to
myotubes
,
(48)
Multilineage commitment of Sca-1(+) cells in reshaping vein grafts
Theranostics
January 1, 2023
Ni Z, Lyu L, Gong H, Du L, Wen Z, Jiang H, Yang H, Hu Y, Zhang B, Xu Q,
et al
.
Briefly, the aortic
adventitia
was harvested and flattened on a flaks coated with 0.04% gelatin (
Sigma
,
G1393
) and cultured in complete
stem cell
culture medium, consisting DMEM (American Type Culture Collection,
(49)
Relative Importance of Different Elements of Mitochondrial Oxidative Phosphorylation in Maintaining the Barrier Integrity of Retinal Endothelial Cells: Implications for Vascular-Associated Retinal Diseases
Cells
December 19, 2022
Shaimaa Eltanani, Thangal Yumnamcha, Andrew Gregory, Mahmoud Elshal, Mohamed Shawky, Ahmed S. Ibrahim
aspiration. The cysteine-coated electrode array was additionally coated with 50 µL of 0.02% gelatin (
Sigma
;
G1393
, Burlington, MA, USA) for another 30 min, followed by aspiration. Then, HRECs were cultured in full
(50)
Protocol to isolate and culture primary mouse feto-placental endothelial cells
STAR Protocols
December 16, 2022
Ionel Sandovici, Moritz Reiterer, Miguel Constância, Cristina M. Branco
Propidium iodide solution
,
Merck
,Cat#P4864
Trypan blue solution
,
Merck
,Cat#
T8154
Accutase
,
Merck
,Cat#
A6964
Gelatin solution
,
Merck
,Cat#
G1393
Formaldehyde solution,Merck,Cat#F1635
TWEEN 20
,
Merck
,Cat#P9416
ProLong gold antifade mountant
,
Thermo Fisher Scientific
(51)
Generation of 2C-like mouse embryonic stem cells in vivo to evaluate developmental potency
STAR Protocols
December 1, 2022
Dennis EK Tan, Baojiang Wu, Siqin Bao, Wee-Wei Tee
Reagent,Final concentration,Amount 2% Gelatin (
Sigma
#
G1393
-100ML),0.1% (w/v),45 mL Sterile water,–,855 mL 0.1% Gelatin solution Filter sterilize through a
(52)
Analysis of sub-kilobase chromatin topology reveals nano-scale regulatory interactions with variable dependence on cohesin and CTCF
Nature Communications
December 1, 2022
Abrar Aljahani, Peng Hua, Magdalena A. Karpinska, Kimberly Quililan, James O. J. Davies, A. Marieke Oudelaar
mouse
embryonic
stem
(mES) cells were cultured on plates pre-coated with 0.1% gelatin (
Sigma-Aldrich
,
G1393
) in Glasgow Modified Essential Medium (
Gibco
,
11710035
) supplemented with 10% fetal bovine serum (Gibco,
(53)
Simultaneous mapping of 3D structure and nascent RNAs argues against nuclear compartments that preclude transcription
Cell Reports
November 29, 2022
Isabel N. Goronzy, Sofia A. Quinodoz, Joanna W. Jachowicz, Noah Ollikainen, Prashant Bhat, Mitchell Guttman
conditions All
mouse
ES cells were grown at 37°C under 7% CO2 on plates coated with 0.2% gelatin (
Sigma
,
G1393
-100ML) and 1.75 μg/mL laminin (
Life Technologies
Corporation, #
23017015
) in serum-free 2i/LIF media
(54)
A minimal-complexity light-sheet microscope maps network activity in 3D neuronal systems
Scientific reports
November 28, 2022
Wysmolek PM, Kiessler FD, Salbaum KA, Shelton ER, Sonntag SM, Serwane F.
Bedzhov, MPI Münster) were maintained under 5% CO2 and 37 °C conditions on 10% gelatine-coated (
Sigma
,
G1393
) T25 flasks in DMEM high glucose medium (Sigma D5671) containing 15% FBS (
Sigma
S0615), 100 U/ml
Penicillin-Streptomycin
,
(55)
On-microscope staging of live cells reveals changes in the dynamics of transcriptional bursting during differentiation
Nat Commun
November 4, 2022
D. M. Jeziorska, E. A. J. Tunnacliffe, J. M. Brown, H. Ayyub, J. Sloane-Stanley, J. A. Sharpe, B. C. Lagerholm, C. Babbs, A. J. H. Smith, V. J. Buckle,
et al.
and leukaemia inhibitory factor (LIF, Cell Guidance Systems, GFM200) on 0.1% gelatin-coated (
Sigma
,
G1393
) tissue-cultured treated plasticware. A detailed protocol on mES
cell differentiation
to produce
erythroid cells
(56)
Individual Variabilities in Adipose Stem Cell Proliferation, Gene Expression and Responses to Lipopolysaccharide Stimulation
International Journal of Molecular Sciences
October 19, 2022
Rumana Yasmeen, Quynhchi Pham, Naomi K. Fukagawa, Thomas T. Y. Wang
at a density of 1.0 × 105/mL were plated in 24-well plates pre-coated with 0.1% gelatin (
Sigma
, Cat #
G1393
) according to manufacturer’s instructions to prevent cells from detaching. Adipogenic differentiation
(57)
Hypoxia induces an early primitive streak signature, enhancing spontaneous elongation and lineage representation in gastruloids
Development
October 15, 2022
Natalia López-Anguita, Seher Ipek Gassaloglu, Maximilian Stötzel, Adriano Bolondi, Deniz Conkar, Marina Typou, René Buschow, Jesse V. Veenvliet, Aydan Bulut-Karslioglu
Berlin, Germany) were cultured without feeders on gelatin-coated plates (0.1% gelatin,
Sigma-Aldrich
,
G1393
). The media was changed every day and cells were passaged every 2 days. At each passage, cells were
(58)
Competition for endothelial cell polarity drives vascular morphogenesis in the mouse retina
Developmental cell
October 10, 2022
Barbacena P, Dominguez-Cejudo M, Fonseca CG, Gómez-González M, Faure LM, Zarkada G, Pena A, Pezzarossa A, Ramalho D, Giarratano Y,
et al
.
μ-Slide I 0.4 Luer (iBIDI, 80176), previously coated with 0.2% gelatin solution in H2O (
Sigma-Aldrich
,
G1393
), 4h prior flow application. When
transfected
cells were used, they were plated 68h after
transfection
.
(59)
Independent regulation of mitochondrial DNA quantity and quality in Caenorhabditis elegans primordial germ cells
eLife
October 6, 2022
Aaron Z A Schwartz, Nikita Tsyba, Yusuff Abdu, Maulik R Patel, Jeremy Nance
8.0], 2.5 mM MgCl2, 0.45% IGEPAL [
Sigma
I8896
], 0.45%
Tween 20
[
Sigma
P9416], 0.01% gelatin [
Sigma
G1393
], and 200 µg/mL
proteinase K
[
Invitrogen
2530049] and flash frozen at –80°C for 15 min). Worms were
(60)
FOLFOXIRI Resistance Induction and Characterization in Human Colorectal Cancer Cells
Cancers (Basel)
September 30, 2022
George M. Ramzy, Laura Boschung, Thibaud Koessler, Céline Delucinge-Vivier, Mylène Docquier, Thomas A. McKee, Laura Rubbia-Brandt, Patrycja Nowak-Sliwinska
a mixture of DMEM/RPMI
1:1
for ECRF24 cells. The latter were seeded on 0.2% gelatin-coated surface (
G1393
,
Sigma-Aldrich
). All culture media were supplemented with 10% fetal bovine serum (S1810-500, Biowest,
Page
<<
<
1
2
3
4
5
>
>>
Reviews
Be the First to Write a Review!
Gelatin solution from MilliporeSigma
Supplier Page
View Company Product Page
Pricing Info
Product Specs
Company Info
Citations
Reviews
Back To Top